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Fruit veg Fruit veg boxes
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This is your one stop, seasonal fruit veg box. A mix of handy weekly staples and unique seasonal gems to keep you cooking up a storm in the kitchen.
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In the next week's box 16 Jul

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Figure 5 | RNA splicing Introns are spliced from the pre-messenger RNA to give messenger RNA (mRNA).

In alternative splicing, individual exons are either spliced or included, giving rise to several different possible mRNA products. Each mRNA product codes for a different protein isoform; these protein isoforms differ in their peptide sequence and therefore their biological activity. It is estimated that up to 60% of human gene products undergo alternative splicing. Several different mechanisms of alternative splicing are known, two of which are illustrated in Figure 6 .

Figure 6 | Alternative splicing Several different mechanisms of alternative splicing exist − a cassette exon can be either included in or excluded from the final RNA (top), or two cassette exons may be mutually exclusive (bottom).

Alternative splicing contributes to protein diversity − a single gene transcript (RNA) can have thousands of different splicing patterns, and will therefore code for thousands of different proteins: a diverse proteome is generated from a relatively limited genome. Splicing is important in genetic regulation (alteration of the splicing pattern in response to cellular conditions changes protein expression). Perhaps not surprisingly, abnormal splicing patterns can lead to disease states including cancer.

In reverse transcription, RNA is "reverse transcribed" into DNA. This process, catalyzed by reverse transcriptase enzymes, allows retroviruses, including the human immunodeficiency virus (HIV), to use RNA as their genetic material. Reverse transcriptase enzymes have also found applications in biotechnology, allowing scientists to convert RNA to DNA for techniques such as .

The mRNA formed in transcription is transported out of the nucleus, into the cytoplasm, to the ribosome (the cell's protein synthesis factory). Here, it directs protein synthesis. Messenger RNA is not directly involved in protein synthesis − transfer RNA (tRNA) is required for this. The process by which mRNA directs protein synthesis with the assistance of tRNA is called translation .

The ribosome is a very large complex of RNA and protein molecules. Each three-base stretch of mRNA (triplet) is known as a codon , and one codon contains the information for a specific amino acid. As the mRNA passes through the ribosome, each codon interacts with the anticodon of a specific transfer RNA (tRNA) molecule by Watson-Crick base pairing. This tRNA molecule carries an amino acid at its 3′-terminus, which is incorporated into the growing protein chain. The tRNA is then expelled from the ribosome. Figure 7 shows the steps involved in protein synthesis.

Figure 7 | Translation (a) and (b) tRNA molecules bind to the two binding sites of the ribosome, and by hydrogen bonding to the mRNA; (c) a peptide bond forms between the two amino acids to make a dipeptide, while the tRNA molecule is left uncharged; (d) the uncharged tRNA molecule leaves the ribosome, while the ribosome moves one codon to the right (the dipeptide is translocated from one binding site to the other); (e) another tRNA molecule binds; (f) a peptide bond forms between the two amino acids to make a tripeptide; (g) the uncharged tRNA molecule leaves the ribosome.

Figure 8 | Two-dimensional structures of tRNA (transfer RNA) In some tRNAs the DHU arm has only three base pairs.

Each amino acid has its own special tRNA (or set of tRNAs). For example, the tRNA for phenylalanine (tRNAPhe) is different from that for histidine (tRNAHis). Each amino acid is attached to its tRNA through the 3′-OH group to form an ester which reacts with the α-amino group of the terminal amino-acid of the growing protein chain to form a new amide bond (peptide bond) during protein synthesis ( Figure 9 ). The reaction of esters with amines is generally favourable but the rate of reaction is increased greatly in the ribosome.

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Experience an enchanting getaway with the whole family in the heart of Europe. Prague is the perfect city to meet everyone’s expectations and to be even more memorable, we are pleasedto welcome you here at the Augustine, a Luxury Collection Hotel, Prague with special treats:

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ft m
From EUR 265

25 - 40 m 2

Maximum Adults/Children
Adults 3
Children 0
From EUR 305

29 - 48 m 2

Maximum Adults/Children
Adults 3
Children 0
From EUR 365

35 - 47 m 2

Maximum Adults/Children
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Children 0
From EUR 415

42 - 57 m 2

Maximum Adults/Children
Adults 3
Children 0
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50 - 59 m 2

Maximum Adults/Children
Adults 3
Children 0
From EUR 965
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Adults 3
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From EUR 1265

70 - 80 m 2

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